질량 분석기: 프로테오믹스 분석을 위한 필수 도구

### 프로테오믹스 질량 분석의 기초
* 🔍 "현대 단백질체학에서 질량 분석기는 **복잡한 단백질체를 합리적인 시간 내에 전체적으로 분석**할 수 있게 해주는 핵심 기기입니다."
* 주기율표의 각 원소는 고유한 질량을 가지며, 이러한 원소로 이루어진 화합물도 **고유한 질량**을 가질 수 있습니다.
* ⚖ 질량 분석기는 이온의 질량을 측정하며, **펩타이드의 질량은 각 아미노산의 질량을 합산**하여 그 서열로부터 계산할 수 있습니다.
* 🔬 질량 분석기는 **이온 소스, 질량 필터, 검출기**의 세 가지 기본 부품으로 구성되며, 이러한 부품의 다양한 조합을 사용하여 특정 유형의 질량 분석기를 만들 수 있습니다.
* 📊 질량 분석기의 출력은 스펙트럼이며, **각 피크는 동일한 분자의 누적된 신호**를 나타냅니다.
* 🎯 "이제 질량 분석기는 **인접한 질량 피크 사이의 1/2 달톤 이하의 차이**를 찾는 선택 장치로 사용하여 추가 분석을 위해 이온을 선택하도록 프로그래밍할 수 있습니다."
* 🔵 프로테오믹스에 사용되는 두 가지 일반적인 이온 소스는 **매트릭스 보조 레이저 탈착 이온화(MALDI)**와 **일렉트로 스프레이**이며, 각각 고유한 장점과 응용 분야가 있습니다.
* 🔄 이온을 압축하는 또 다른 방법은 **전기 반사판**을 사용하는 것입니다. 이 방법은 두 가지 기능을 수행합니다. 첫째, **비행 경로를 더 길게 만들어 질량이 약간 다른 이온이 시간적으로 더 잘 분리**되도록 합니다. 둘째, 같은 질량의 이온을 공간과 시간에 따라 함께 압축하여 **질량이 급격히 피크**가 되는 결과를 낳습니다.
* 🔒 4중극자 질량 필터는 이온 선택에 자주 사용되며, **삼중 4중극자 구성은 선택적 반응 모니터링(SRM) 분석**에 사용됩니다.
* 🔄 **4중극자 비행 시간(Q-TOF) 및 4중극자 오비트랩**과 같은 하이브리드 질량 분석기는 여러 질량 분석기를 결합하여 다양한 유형의 분석에 각 분석기의 장점을 활용합니다.

질량 분석기는 이온의 질량 대 전하 비율을 측정하여 복잡한 프로테옴의 고유한 질량 분석을 가능하게 하므로 프로테오믹스 분야에서 매우 중요합니다.

in modern proteomics, the mass spectrometer is the key piece of instrumentation that allows the global analysis of complex proteome to happen in a reasonable timeframe. This presentation is all about how mass spectrometers actually work because you need to understand how an instrument generates data before you can properly interpret the data. The first question is why is math so important for identifying proteins. Each element in the periodic table has a unique mass. In this table listing the masses of the main elements making up organic compounds such as proteins, it can be seen that each element and the isotopes of those elements have a unique mass. The difference can be averaged to one Dalton, or the mass of a proton or neutron. Carbon has three isotopes which vary by the number of neutrons in their nuclei, but otherwise they are chemically identical. The carbon-13 isotope is only a very small proportion of the total carbon on the planet, but it is extremely important for mass spectrometry, as we will see. Because elements have unique masses, compounds made of those elements

can also have unique masses for ethanol. When the masses of the elements making up ethanol are summed, the compound molecular mass can be calculated. This can be repeated for carbohydrates or other organic molecules, such as this toxin from potatoes, and more importantly, proteins and peptides. The mass of a peptide can be calculated from its sequence by summing together the masses of each amino acid. But what if we don't know the sequence of the peptide? This is where the mass spectrometer becomes useful. Mass spectrometers measure the mass of ions, which are charged particles. So, unless you can either add a hydrogen atom to an amine or remove a hydrogen from the carboxyl group of a peptide, you cannot measure its mass. Mass spectrometers consist of three basic parts that can be put together in different combinations to make specific types of mass spectrometers that are useful for specific types of analyses. First, you need something to generate the ions, called the ion source. Then, you need something to measure the mass of the ions, sometimes called a mass filter.

combinations of mass filters can be put together for different purposes. Lastly, you need something to detect and count the ions. The mass filter and detector are always under vacuum so that random molecules floating around in the air don't get measured. Once all of this happens, a spectrum is generated. In this case, for solanum, the spectrum shows a number of populations of molecules, with each peak being the accumulated signal of hundreds to thousands of molecules of exactly the same thing. The major peaks show the molecules of solanum whose carbon atoms are all the C12 isotope. The next peak appearing at one dot and larger other molecules of solanum that have one carbon atom with an extra neutron, the C-13 isotope. In different molecules, the C-13 atom can be at a different place in the structure, but the mass of the intact compound will be the same. Thus, we can see the accuracy of the mass spectrometer that can measure the difference in mass 2 less than the mass of a hydrogen atom.

difference in mass between the C12 and C-13 isotopes of ions is important when measuring peptides. Mass spectrometers don't measure mass but mass divided by charge ratios or M over Z. So for Salonen, which takes up a single proton to become an iron, the mass over charge ratio for the C12 iron is 868 and 1/2 divided by 1 charge, and the C-13 isotope with that extra Neutron is 869 and 1/2 divided by the one charge. Thus, the difference in mass between these isotopes is 1 Dalton, reflecting the mass of the neutron. Peptides such as this one, because they have two primary amines, can take up 2 hydrogen atoms and thus have 2 charges. The calculated mass of this peptide sequence is fifteen hundred and seventy Daltons, but the mass spectrometer measures the mass / charge ratio for the C12 isotope of 785 point 8. This is because it is measuring the mass of the peptide plus two hydrogen atoms divided by the

two charges of those hydrogen atoms the c-13 isotope containing the extra Neutron of the peptide is measured as 786 point 3 or 1/2 adult and larger then rather than one Dalton. Because the mass spectrometer is measuring the mass of the peptide plus a neutron plus two hydrogen atoms and then dividing it by two charges. The mass spectrometer can now be programmed to use this as a selection device looking for 1/2 Dalton or less differences between adjacent mass Peaks to select ions for further analysis. Now that we've got that sorted out it's time to look at the ways of getting peptides into the mass spectrometer or looking at the ion sources. The first we will look at is matrix-assisted laser desorption ionization or maui invented in the mid-1980s by Franz Helen camp and Michael Karras. In this technique the peptides are spotted onto a metal target plate with an ultraviolet light absorbing compound called a matrix and allowed to dry. The two main matrices use

our alpha-cyano-cinnamic acid or CHCA, and cinnamic acid. The plate is then put into the mass spectrometer, and a high voltage is applied to the plate, about fifteen kilovolts. A UV laser is then fired at the sample, and energy absorbed by the matrix, which transfers this energy to the peptide molecule. This ionizes the peptide molecules off the target plate, and they are accelerated past a counter electrode at twelve kilovolts into the mass filter, which is normally a time-of-flight detector. It is important to note that MALDI predominantly generates singly charged ions, not multiply charged. Time-of-flight is the simplest form of mass filter, measuring the time it takes for an ion to travel from one end of a tube to the other end and strike a detector. The laser pulse starts the clock, and an ion striking the detector is the measure of time. The larger the ion, the slower it travels. The mass can then be calculated by inserting the flight time into this equation. Essentially, it's like measuring how long

it takes for something to get from slingshot to target. As with everything else we've discussed, we are not measuring a single iron of a particular mass, but hundreds to thousands of molecules of the same identical peptide. When the plume of ions is first produced, they are spread out a little in space and thus time. At the detector, the number of ions hitting the detector is measured in bins of half a nanosecond. After a specified time range has been scanned, we can connect the intensities together to draw a peak, the apex of which is the mass of an ion. All of these intensity lines are the result of the same mass ion hitting the detector but at slightly different times over a few nanoseconds. Thus, to increase the accuracy of the mass measurement and increase sensitivity, we want all of the ions to hit the detector at the exact same time, making the peak as narrow as possible. The first place to compress the ions into a smaller space is at the ion source using a technique called delayed extraction where electrical pulses are

used to allow lagging ions to catch up before entering the time-of-flight tube. Another way of compressing ions is to use an electrical reflector. This uses an electrical mirror to reflect the ions back to a detector. This does two things: firstly, it makes the flight path longer so that ions of slightly different masses are better separated in time. Secondly, it compresses the ions of the same mass together in space, and thus time, resulting in a sharp peak for a mass. The difference between a linear TOF and a reflector TOF can be seen here. These are both the same set of peptides, the lower trace being linear TOF and the upper trace being the reflector TOF. The insets are a zoom on the same specific peak. In the linear TOF, none of the isotopic forms of the peptide can be resolved, whereas the reflector TOF is able to resolve the C12 isotope from the C13 isotope with ease. The alternate form of ion source is electrospray, again invented in the 1980s.

by John Fenn. In electrospray, the peptidyl protein is in a liquid that flows out of a capillary that is under high voltage, usually between 1,500 and 5,000 volts, depending on the flow rate of a liquid. With a lower flow requiring lower voltages for peptides and proteins, the liquid is normally an acidified mixture of water and organic solvents such as acetonitrile. The acidic conditions mean that there is an excess of hydrogen ions in solution, and the peptide molecules become protonated on their primary amines, as previously described. As the liquid is sprayed, it forms a plume of droplets that rapidly evaporate, making the droplets smaller and smaller, and pushing the positively charged peptides together closer. The molecules of the same charge don't like being too close together, so there is a point where the charge density becomes too high (the Rayleigh limit), and the droplet explodes, leaving the ions to accelerate into the mass analyzer, often a quadrupole, that can be placed before a

time-of-flight detector electrospray generates multiple charged ions as seen in this example of lysozyme which has an intact mass of about fourteen thousand Dalton's. As mass spectrometers measure mass divided by charge ratios, the iron masses here show a population of lysozyme ions with either 7 charges, 8 charges, 9 charges, ten charges, and so on and so forth. To determine the mass of the lysozyme iron with one charge and thus its true mass, we can use simultaneous equations. Computer algorithms exist to perform this task much faster. Thus, electrospray can be used to very accurately measure the mass of a protein using a mass analyzer with a relatively small mass range, which makes the mass spectrometer more sensitive. To illustrate the main difference between Maori and electrospray, we can observe these two spectra of myoglobin, a protein with a mass of sixteen thousand nine hundred and fifty-two Dalton's. In the upper spectra from the from Mao D, we see the singly charged iron at sixteen

thousand nine hundred and fifty-two and an iron of myoglobin with two charges. At half of that mass in the electrospray spectra, we can see the multiple charge states of different populations of myoglobin Enza ions. The main advantage of using electrospray is that it can be interfaced with liquid chromatography more easily than Maori, but we'll discuss this further when we have discussed chromatography in more detail. Electrospray is most often interfaced with quadrupole mass filters, although iron traps were very popular for a long time. We will discuss those briefly later. A quadrupole consists of four rods arranged in a square that can have different amounts of voltage or radiofrequency applied to them. At a certain voltage and radio frequency, a certain mass to charge ratio iron will be passed through the quadrupole to a detector while other ions are lost to the vacuum system. To scan a mass range, the voltage and radio frequency must be varied, and so sensitivity is reduced. So why would you use a quadrupole Quadra

files are fantastic at selecting specific mass-to-charge ratio ions and transmitting them to other types of mass analyzers. One configuration is the triple quadrupole, shown in this diagram. The first quadrupole is used for ion selection, for selection of a specific ion with all others being excluded from the quadrupole. The selected ion is then transmitted to the second quadrupole, which is a collision cell. This cell is filled with nitrogen gas molecules, and the ions are accelerated into the collision cell where they collide with the nitrogen molecules and get smashed into fragments. The fragments then get transmitted to the third quadrupole, which is set to transmit only one fragment to the detector, while the rest are lost. The result of this is an extremely sensitive and specific assay called selected reaction monitoring, which can be thought of as doing a Western blot with a mass spectrometer. It requires that you know the identity of the peptides that you want to measure to program the mass spectrometer. In other words, you can target specific peptides in a complex mixture. These spectra

represent full scans of the intact peptides selected by quadrupole one, and the fragment peptides selected by quadrupole three. However, the output is a time-based chromatogram showing when the ion selected by Q three is transmitted and is detected. This can only happen when the intact peptide selected by Q one is transmitted to the collision cell for fragmentation, and a specific fragment is transmitted by Q three to the detector. These combinations of Q one mass and Q three mass are referred to as a transition. The mass spectrometer can be set to scan transition combinations for about 50 milliseconds before moving on to a different combination for another 50 milliseconds. Thus, a list of a few hundred transitions and peptides can be scanned through over a few seconds, allowing numerous proteins to be assayed in one experiment, rather than one at a time for a Western blot. But in the majority of proteomics experiments, we do not know the identity of the peptides or proteins, so we need to operate the mass spectrometer in a discovery mode using

scans of a mass range, as mentioned. Quadra holls do this poorly with low sensitivity and resolution, so hybrid instruments are often used. One of the most popular is the quadrupole time-of-flight hybrid, which is shown in this diagram. It consists of the front two quadrupole zuv, a triple quadrupole instrument, the q1 for iron selection, and the collision cell for measuring for fragmenting peptides. The third quadrupole is replaced with the time-of-flight mass analyzer. As mentioned earlier, the time of flight requires a start time to measure the ions' flight time. But using chromatography to produce ions at the source means that ions are continuously flowing through the quadrupole. We create a start time by making the ions turn 90 degrees into the toff every 250 milliseconds. The pusher pulses a packet of ions into the toff. The ions fly down to a reflector and are reflected back to the detector. Thus, we can scan a mass range and get high-resolution data. This animation shows things in more detail.

all of these balls represent ions. The grey ones represent uncharged ions that will get sucked out by the vacuum system. The positively charged ions will continue through from the sampling capillary through a series of electrical lenses into the quadrupole. In the quadrupole, here the only iron being selected is the green one. All of the rest are excluded by the vacuum system. The green iron has been selected and then transmitted to the collision cell where it will collide with nitrogen gas molecules to produce fragments. You can see the nitrogen gases, the hazy things here, and you can see the fragmentation of these green ions occurring. The fragments then get transmitted out the other end of the collision cell to the time of flight tube. The fragment ions are compressed into a nice flat packet before they reach the top tube so that all of the ions have the same starting time before they are pulsed into the top. As you can see them rising straight up, they all have the same start time, and ions of the same mass stay together, but ions of different

masses have a different flight time. They get reflected back from the reflector on and down to the detector. They strike the detector and cause a cascade of ions, which is then measured by a photomultiplier tube. Thus, different masses are detected in bins. There are not many uses of cyclotrons nowadays because the problem with them is that they're very expensive to run, as the magnets require cooling with liquid helium. In proteomics applications, they have been replaced with an alternative type of cyclotron called an orbitrap, which does not have a magnet. In an orbitrap, the ions circle or orbit around a central spindle, again inducing current in collection plates, which is then interpreted by Fourier transform to produce a mass spectrum. More detail on how these works can be found in this video from Thermo. As with quadrupole TOFs, orbitraps are most often used with other mass analyzers as a hybrid. The original instruments produced were paired with ion traps, which we are not going to discuss any further except to say that they are able to trap specific

ions and fragment them in the same space. Unlike a quadrupole, which transmits ions to another quadrupole for fragmentation, recent Orbitrap-based instruments use the Orbitrap for scanning a mass range to measure intact peptide mass before using a quadrupole to select a specific ion and fragmenting that ion in a separate collision cell before transmitting the fragment ions back to the Orbitrap for measurement. For proteomics purposes, either a quadrupole TOF-based or an Orbitrap-based mass spectrometer would give good data, but specific differences can make one type more useful for certain types of analyses. While the Orbitrap incorporates a detector by inducing a current in detector plates, triple quads, quad TOFs, and ion traps need detectors after the mass analyzer. Triple quads and ion traps use dynodes where a charged ion hits an electrode, creating a secondary electron that increases or amplifies the signal. The mass is determined by the software knowing what the voltage and the frequency of the quadrupole was.

those values determine a certain mass time of Fey analyzes. Time-of-flight analyzers are coupled with microchannel plates. A charged iron hits the front of the plate and causes a cascade of electrons to hit an electrode, resulting in signal amplification. The mass is determined by measuring the time from the start signal coinciding with the ions being pulsed by the pusher and the ions of a single mass hitting the detector. We revisit this diagram from earlier where ions arriving are counted in bins of half a nanosecond after enough time has passed to measure the desired mass range, typically 350 to $1,500. The intensity bars are joined to produce a spectra. This concludes our very basic introduction of how mass spectrometers are used in proteomics work. In the next presentation, we will discuss how the instruments can be used to solve questions in proteomics.

Mass spectrometry for proteomics - part one

Matt Padula

현대 단백체학에서 질량 분석기의 역할

질량 분석기는 단백질과 펩타이드를 식별하기 위해 이온의 고유 질량을 측정하기 때문에 복잡한 프로테옴을 전체적으로 분석하는 데 매우 중요합니다.

원소와 그 동위원소의 고유한 질량을 통해 유기 분자와 단백질을 포함한 화합물의 분자 질량을 계산할 수 있습니다.

질량 분석기는 이온 소스, 질량 필터, 검출기로 구성되며, 모두 진공 상태에서 작동하여 분자와 그 동위 원소의 집단을 보여주는 스펙트럼을 높은 정확도로 생성합니다.

펩타이드 측정에서 C12 및 C-13 동위원소의 중요성

질량 분석에서 펩타이드를 측정할 때 C12와 C-13 동위원소의 질량 차이는 매우 중요한데, 1달톤의 차이는 중성자의 질량을 반영합니다.

질량 분석기는 이러한 동위 원소의 질량/전하 비율을 측정하여 질량 차이에 따라 추가 분석을 위한 이온을 선택할 수 있습니다.

1980년대 중반에 발명된 매트릭스 보조 레이저 탈착 이온화(MALDI)는 펩타이드 분자를 이온화하는 데 사용되는 기술로, 비행 시간 질량 필터에서 분석을 위해 주로 단일 하전 이온을 생성합니다.

질량 분석 정확도 및 감도 극대화

질량 분석은 정확도를 높이기 위해 모든 이온이 검출기에 정확히 동시에 부딪히도록 하는 것을 목표로, 많은 수의 동일한 분자의 질량을 측정하여 피크를 도출합니다.

이는 지연 추출 및 전기 반사와 같은 기술을 통해 이온을 더 작은 공간으로 압축하여 질량에 대한 날카로운 피크를 생성함으로써 달성됩니다.

또 다른 이온 소스 기술인 일렉트로스프레이는 고전압 액체를 사용하여 펩타이드 분자를 양성자화하여 질량 분석기로 가속하여 질량 분석의 정확도와 감도를 극대화합니다.

질량 분석에서 일렉트로스프레이의 역할

일렉트로스프레이는 단백질에 대해 여러 하전 이온을 생성하는 데 사용되며, 동시 방정식 또는 컴퓨터 알고리즘을 사용하여 정확한 질량 측정이 가능합니다.

액체 크로마토그래피와의 인터페이스에 유리하며, 특히 삼중 사중극자 구성에서 사중극자 질량 필터와 함께 일반적으로 사용됩니다.

이를 통해 복잡한 혼합물에서 특정 펩타이드를 표적으로 삼는 데 사용되는 선택적 반응 모니터링으로 알려진 매우 민감하고 구체적인 분석이 가능합니다.

4중극자 비행 시간 질량 분석법을 이용한 펩타이드 분석 과정

4중극자 1은 온전한 펩타이드를 선택하고, 4중극자 3은 단편 펩타이드를 선택하며, 이 두 가지의 조합을 전이라고 합니다.

질량 분석기는 몇 초 안에 수백 개의 전이를 스캔할 수 있으므로 한 번의 실험으로 수많은 단백질을 분석할 수 있습니다.

4중극자 비행 시간 하이브리드 질량 분석기는 이온 비행 시간을 측정하기 위한 시작 시간을 생성하는 고유한 방법을 사용하여 높은 감도와 분해능을 제공합니다.

질량 분석에서 사이클로트론 및 오비트랩 사용

사이클로트론은 냉각을 위해 자석과 액체 헬륨을 사용하는 반면, 오비트랩은 자석 없이 작동하므로 보다 비용 효율적인 대안을 제공합니다.

오비트랩은 종종 4중극자 TOF와 같은 다른 질량 분석기와 함께 프로테오믹스 분야에 사용됩니다.

트리플 쿼드 및 이온 트랩을 포함한 질량 분석 기기는 신호 증폭 및 질량 측정을 위해 다이노드 및 마이크로채널 플레이트와 같은 다양한 유형의 검출기를 활용합니다.